Making assemblies for Oxford Nanopore sequence data using Dragonflye

I've been making genome assemblies for some Oxford Nanopore Technology (ONT) sequencing data using the Dragonflye package by Robert A. Petit III. 

It was super easy to run!

Here's how I ran it on the Sanger compute farm:

First I found the version of Dragonflye on the farm:

% module avail -t | grep -i dragon

Then I loaded it:

% module load dragonflye/1.2.1

Then I assembled sequence reads for a Vibrio cholerae isolate into an assembly using Dragonflye: 

%  dragonflye --reads SRR31024125_1.fastq.gz --outdir SRR31024125_1.fastq_dragonflye --gsize 4000000

where  SRR31024125_1.fastq.gz was my input fastq file of ONT reads,

SRR31024125_1.fastq_dragonflye was the name that I wanted to give to the output directory,

 --gsize 4000000 specifies that the Vibrio cholerae genome is about 4.0 Mbase.

The output file was called SRR31024125_1.fastq_dragonflye/contigs.fa.  

It took about 20 minutes to make the assembly. The input file of ONT reads was about 93 Megabytes (SRR31024125_1.fastq.gz).